ZFIN Image: Hsieh et al., 2021, Figure 5

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Figure Caption

Figure 5

Effects of α-MSH on feeding regulation in the hypothalamus of zebrafish. (A,B) RT–qPCR analysis was used to measure the mRNA levels of four selected orexigenic genes, pmch, agrp2, npy, and hcrt (A), and five selected anorexigenic genes, bdnf, sim1a, crhb, trh, and pcsk2 (B) in the WT and pomca mutants at 1.5 h after feeding. (C) Whole-mount ISH showing decreased expression of bdnf and sim1a in the pituitary in WT and pomca mutant larvae at 5 dpf. Scale bars, 200 μm. (D) RT–qPCR analysis was used to measure the mRNA level of the intestinal orexigenic gene, ghrl, in WT and pomca mutant adults. Values are means ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with WT control groups.

Acknowledgments

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