FIGURE

Figure 4

ID
ZDB-FIG-230629-60
Publication
Mazumdar et al., 2023 - Loss of Nicotinamide nucleotide transhydrogenase sensitizes embryos to ethanol-induced neural crest and neural apoptosis via generation of reactive oxygen species
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Figure 4

Severity of nnt malformations increases with ethanol concentration. (A–L) Untreated and ethanol-exposed Alcian Blue/ Alizarian Red-stained zebrafish embryos at 5 dpf treated from 6 hpf- 24 hpf. All images are ventral views with anterior to the left. (A,B,G,H) Wildtypes and mutants appear phenotypically normal at concentrations of 0 and 0.5% ethanol. (C,I) At 0.75% ethanol, wildtypes still appear normal, but 22.7% of mutants have ceratohyal cartilages that do not extend anteriorly (red arrow). (D,J) At 1% ethanol, the wildtypes remain normal, but 82.2% of nnt mutants had gross craniofacial defects, including a hypoplastic Meckel’s cartilage (yellow arrow). (E,F,K,L).. At 1.25 and 1.5% craniofacial defects become apparent in the wildtypes, and there is increased severity of the craniofacial defects in the mutants. (M) A bar chart depicting total craniofacial malformations across genotype and ethanol concentration (Fisher’s exact test, n ≥ 20 per group, *p  < 0.05, ****p  < 0.0001). (N) A bar chart depicting the types of craniofacial malformations seen in mutants at different ethanol concentrations. EtOH: ethanol, Mut: mutant, Het: heterozygote, Wt: wildtype.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage: Day 5

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neurosci.