FIGURE

Fig. 4

ID
ZDB-FIG-230328-35
Publication
Ellis et al., 2022 - A missense mutation in the proprotein convertase gene furinb causes hepatic cystogenesis during liver development in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

The mutant Furinb protein acts within the biliary cells to disrupt bile duct morphogenesis. (A) Schematic diagram of the genetic mosaic analysis for determining the cell autonomy of the mutant Furinb function. (B,C) Representative results of the genetic mosaic analysis. (B) WT donor cells transplanted into the s741 mutant host liver (6 of 6 host fish exhibited the representative phenotype). (C) s741 mutant donor cells transplanted into the WT host liver (10 of 10 host fish exhibited the representative phenotype). In both (B) and (C), the WT biliary cells expressed Tg(Tp1:ras‐mCherry) (red) and the s741 mutant biliary cells expressed Tg(Tp1:GFP) (green). (D–I) Confocal 3D projections of larvae stained with biliary marker Anxa4. Ventral view; anterior is on the top. (J,K) Numbers (mean ± SEM) of cysts per liver (J) and percentages of livers with cysts based on Anxa4 staining (K). In (J), each dot represents individual liver. Statistical significance was calculated by one‐way ANOVA and Tukey's post‐hoc test (J), and calculated by Fisher's exact test (J): *p < 0.05; **p < 0.01, ***p < 0.001; ****p < 0.0001. Scale bars: 30 μm (B,C) and 40 μm (D–I).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Hepatol Commun