FIGURE

Fig. 4

ID

ZDB-FIG-230115-74

Publication

Lu et al., 2021 - Establishment of a Ciliogenesis-Associated Signaling Model for Polycystic Kidney Disease

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Fig. 4

Functional verification of PI4KB for PKD. A The PKD phenotypes generated by PI4KB mutants. The asterisk indicates dilated tubules. B The statistical analysis for PKD phenotypes compared mutants with sibling controls. C The immunostaining of primary cilia for PI4KB mutant zebrafish. The white arrow represents the absence of single cilia in the pronephric duct of mutant zebrafish. D Western blotting and corresponding densitometry analysis demonstrate the expression level of PI4KB in the control vector and GNAS siRNA-transfected HK-2 cells. E, F Western blotting shows the activity change of t-Akt and p-Akt in the control vector, PI4KB siRNA, and GNAS siRNA-transfected HK-2 cells, respectively. The asterisk indicates p < 0.05. t-Akt, total Akt; p-Akt, phospho Akt.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pi4kb^{zf3540/zf3540} pi4kb^{zf3541/zf3541}
Observed In:	pronephric tubule pronephros cilium
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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