FIGURE

Fig. 8

ID
ZDB-FIG-210520-8
Publication
Bertho et al., 2021 - Zebrafish dazl regulates cystogenesis and germline stem cell specification during the primordial germ cell to germline stem cell transition
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Fig. 8

Single confocal plane of representative dazlae57/+ or dazlae57/ae57 10-14-day gonads labeled with fluorescently conjugated phalloidin (actin) depicting the actin ring. Each stage is represented with a sagittal view (XY) or maximum projection (ten planes of a z-stack). (A,A′,C,C′,E,E′) Actin rings are indicated by a blue arrow in dazlae57/+ 8-14-day gonads. Number of gonads analyzed: 10 days, n=3 (A,A′); 12 days, n=3 (C,C′); 14 days, n=3 (E,E′). (B,B′,D, D′,F,F′) Actin rings at 10 days in dazlae57/ae57 cells. At 12- and 14-days, actin rings are not maintained (blue arrows). Gonads analyzed: 10 days, n=4 (B,B′); 12 days, n=2 (D,D′); 14 days, n=3 (F,F′). (G) TEM image of day 14 wild-type gonad stitched from tiled images (magnification 700×). (H) Magnified image of (G, pink dashed line box) showing a ring canal connecting sister GCs within a cyst. (I) Higher magnification of H (pink dashed line box). (J) TEM image of 14-day dazl mutant gonad stitched from tiled images (magnification 700×). No cysts or ring canals were detected. Pink arrows indicate the centrioles and green arrows indicate the ring canal. Blue arrowheads indicate the germ cell membranes.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Days 7-13 to Days 14-20

Phenotype Detail
Acknowledgments
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