FIGURE

Fig. 1

ID
ZDB-FIG-170922-44
Publication
Scahill et al., 2017 - Loss of the chromatin modifier Kdm2aa causes BrafV600E-independent spontaneous melanoma in zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 1

Loss of Kdm2aa function leads to growth and oogenesis defects.

(A) The zebrafish paralogues Kdm2aa and Kdm2ab share the protein domain structure with their human orthologue KDM2A. The mutations kdm2aasa898 and kdm2aasa9360 produce premature stop codons in the JmjC and F-box domain, respectively. The allele kdm2absa1479 produces a premature stop codon in the JmjC domain. (B) Juvenile fish compound heterozygous for kdm2aasa898 and kdm2aasa9360 are shorter and thinner at 30 d.p.f. than their heterozygous siblings. Scale bar: 5 mm. (C) Box plots showing length in mm of each of the three sibling groups for a representative family at 30, 90 and 180 d.p.f. Fish homozygous for kdm2aasa898 are smaller than their siblings. (D) Fish homozygous for premature stop codons in kdm2aa represent less than the expected 25% of the group. (E) A small proportion of maternal zygotic kdm2aasa9360 embryos survive to 5 d.p.f. with mild (arrow in second panel) or no malformations (bottom panel). (F) Embryos derived from kdm2aasa9360/sa9360 intercrosses show severe defects and lethality during the first 24 h.p.f. whereas kdm2aasa9360/sa9360 male outcrosses are normal. (G) Two examples of embryos derived from kdm2aasa9360/sa9360 female outcrosses displaying severe defects at 24 h.p.f. (H) TRITC-Phalloidin and DAPI staining on MZkdm2aa-/- embryos derived from intercrosses of kdm2aasa898/sa9360 fish at the 8–16 cell stage showed that despite cell asymmetry and asynchronous division, nuclei seemed overall normal. (I) Expression data from Expression Atlas http://www.ebi.ac.uk/gxa/experiments/E-ERAD-475 showing normalised counts (FPKMs) from RNA-seq data for kdm2aa and kdm2ab. There is maternal contribution of both genes, but kdm2ab polyA transcript abundance peaks at early blastula stages, whereas kdm2aa polyA transcript abundance peaks later at the gastrula shield stage.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: 8-cell to Adult

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.