FIGURE

Fig. S4

ID
ZDB-FIG-161201-55
Publication
Kantarci et al., 2016 - Spemann organizer gene Goosecoid promotes delamination of neuroblasts from the otic vesicle
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Fig. S4

Heat-shock activation of hs:gsc during gastrulation or otic development. (A–C) Whole-mount live images at 24 hpf (dorsal up, anterior left) show the phenotypic effects of a 30-min, 39 °C heat shock initiated at 6 hpf in control and hs:gsc embryos. Activation of hs:gsc under these standard heat-shock conditions dorsalized the embryos to varying degrees (B and C). (D–I) Whole-mount images (dorsal up, anterior left) showing gsc expression in controls and hs:gsc embryos. Embryos were fixed and stained at indicated times after the end of a 60-min heat shock initiated at 22 hpf. Activation of hs:gsc at 22 hpf led to a strong increase in the gsc level by the end of the heat-shock period (E). The level of gsc expression was maximal at 23.5 hpf (F) but declined rapidly thereafter, returning to normal by 25 and 26 hpf (H and I).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA