FIGURE

Fig. S1

ID

ZDB-FIG-121102-28

Publication

Matsui et al., 2012 - Celf1 regulation of dmrt2a is required for somite symmetry and left-right patterning during zebrafish development

Other Figures

All Figure Page

Back to All Figure Page

Fig. S1

celf1 plays a crucial role in symmetric somitogenesis and LR asymmetric patterning. (A) celf1_long-MO and celf1_short-MO inhibited the expression of celf1L-GFP and celf1S-GFP, respectively. (B) Western blotting using anti-Celf1 antiserum. Left panel: co-injection of celf1_long-MO with celf1_short-MO (celf1-MOs) inhibited the expression of both forms of endogenous Celf1 in zebrafish embryos. The asterisk indicates a non-specific band. Right panel: Celf1 and Celf1^Δlinker proteins were detected in Celf1- and Celf1^Δlinker_overexpressing embryos, respectively. (C) Representative images of myod expression demonstrating normal (left; control-MO, n=105) or abnormal (right; celf1-MOs, n=88) somitogenesis in embryos at 24-28 hpf. Higher magnification images (lower panels) highlight somites. Knockdown of celf1 resulted in a mild loss of the chevron shape of somites (94%). (D) Upregulation of myod expression in celf1 morphants. qPCR assay revealed that celf1 knockdown resulted in a 50% increase of myod expression in zebrafish embryos, suggesting that myod is a target of Celf1 not only in C2C12 (Lee et al., 2010) but also in zebrafish.

Expression Data

Gene:	celf1
Fish:	WT WT + MO1-celf1 + MO2-celf1
Knockdown Reagents:	MO1-celf1 MO2-celf1
Anatomical Term:	whole organism
Stage Range:	5-9 somites to 10-13 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-celf1 + MO2-celf1
Knockdown Reagents:	MO1-celf1 MO2-celf1
Observed In:	somite
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development