FIGURE

Fig. 3

ID
ZDB-FIG-101115-32
Publication
Padmanabhan et al., 2009 - Cardiac and vascular functions of the zebrafish orthologues of the type I neurofibromatosis gene NFI
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Fig. 3

MO knockdown of nf1a, nf1b, or both together results in pericardial effusions at 3.5 dpf and increased phospho-p44/42 MAPK in cardiac tissue. Analysis of 3.5-dpf wild-type embryos (A) or embryos injected with nf1a ATG 5-mispair (5MP) MO (B), nf1b ATG 5MP MO (D), or nf1a + nf1b ATG 5MP MO (F) reveal no apparent defects in gross morphology. Treatment with nf1a ATG MO (C), nf1b ATG MO (E), or a combination of both (G), however, results in a dilation of the pericardial space. (H–J) Injection of p53-/- embryos with nf1a + nf1b ATG MO results in a gross dilation of the pericardial space (J), whereas uninjected (H) and nf1a + nf1b ATG 5MP MO-injected p53-/- embryos (I) appear normal. (Scale bars: 0.25 mm.) (K and L) Transverse sections of 3.5-dpf nf1a/nf1b combined morphant embryos reveals a thinning of the ventricular myocardium and pericardial effusion (*) when compared with controls (A, atrium; V, ventricle). (M and N) Immunohistochemical analysis of transverse sections of 3.5-dpf nf1a/nf1b combined morphant embryos reveals an increase in the ratio of phospho-p44/42 MAPK-positive cardiac cells (arrows) to the total number of cardiac cells when compared with controls. (Scale bars: 25 μm.)

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA