FIGURE

Fig. 5

ID
ZDB-FIG-100802-8
Publication
Zhao et al., 2010 - Klf6/copeb is required for hepatic outgrowth in zebrafish and for hepatocyte specification in mouse ES cells
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

Decreased endoderm-derived organ size in copeb morphants is due to decreased proliferation. (a) Proliferation in whole embryos was examined at 26, 56, and 74 hpf by immunofluorescence using an antibody against phosphorylated Histone H3 (p-histone H3) to identify cells in the G2/M phase of the cell cycle. High levels of proliferation are found in the head, eyes and tails of both morphants and controls; n = 20 for each sample at each time point. (b–d) Hepatocyte proliferation was assessed by nuclear PCNA staining of serial sections of the liver (outlined) in control embryos (b) and copeb morphants (c) on 4 dpf and was quantified in (d). scale bar = 20 microns. At least 1000 hepatocytes in at least 2 control and 3 morphant embryos were scored for each sample.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Day 4

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 344(1), Zhao, X., Monson, C., Gao, C., Gouon-Evans, V., Matsumoto, N., Sadler, K.C., and Friedman, S.L., Klf6/copeb is required for hepatic outgrowth in zebrafish and for hepatocyte specification in mouse ES cells, 79-93, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.