FIGURE

Fig. 1

ID

ZDB-FIG-090714-1

Publication

Weiser et al., 2009 - Rho-regulated Myosin phosphatase establishes the level of protrusive activity required for cell movements during zebrafish gastrulation

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Fig. 1

Myosin phosphatase is required for body axis elongation in zebrafish. (A-F) Dorsal views of 48 hpf zebrafish embryos injected with (A) control, (B) 1 ng mypt1 MO, (C) 200 pg cpi-17 mRNA, (D) 100 pg ca-mlck mRNA, (E) 100 pg of human MYPT1 mRNA. (F) A partially rescued embryo injected with 100 pg human MYPT1 mRNA and 1 ng mypt1 MO. (G,H) Ventral views of the head of 48 hpf zebrafish embryos showing a ca-mlck-injected embryo displaying cyclopia (G) and a control embryo (H). (I) Western blot showing endogenous Mypt1 levels in the presence and absence (WT) of 1 ng mypt1 MO. (J) Quantification of the truncated body axis phenotype in morphant and mRNA-injected embryos. The y-axis displays the percentage of embryos exhibiting a severe axis extension defect (red) or normal axis extension (blue). The x-axis displays the injected reagent.

Expression Data

Gene:	ppp1r12a
Fish:	WIK/AB WIK/AB + MO3-ppp1r12a
Knockdown Reagent:	MO3-ppp1r12a
Anatomical Term:	whole organism
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WIK/AB + MO3-ppp1r12a
Knockdown Reagent:	MO3-ppp1r12a
Observed In:	axis convergent extension involved in axis elongation whole organism
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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