PUBLICATION
Identification of fish CMPK2 as an interferon stimulated gene against SVCV infection
- Authors
- Liu, W., Chen, B., Chen Li, ., Yao, J., Liu, J., Kuang, M., Wang, F., Wang, Y., Elkady, G., Lu, Y., Zhang, Y., Liu, X.
- ID
- ZDB-PUB-190528-5
- Date
- 2019
- Source
- Fish & shellfish immunology 92: 125-132 (Journal)
- Registered Authors
- Keywords
- Antiviral immunity, CMPK2, Interferon-stimulated gene, Spring viraemia of carp virus
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Fish Diseases/immunology*
- Gene Expression Profiling/veterinary
- Gene Expression Regulation/immunology*
- Immunity, Innate/genetics*
- Interferons/metabolism
- Phylogeny
- Rhabdoviridae/physiology
- Rhabdoviridae Infections/immunology
- Rhabdoviridae Infections/veterinary
- Sequence Alignment/veterinary
- Zebrafish/genetics*
- Zebrafish/immunology*
- PubMed
- 31125665 Full text @ Fish Shellfish Immunol.
Citation
Liu, W., Chen, B., Chen Li, ., Yao, J., Liu, J., Kuang, M., Wang, F., Wang, Y., Elkady, G., Lu, Y., Zhang, Y., Liu, X. (2019) Identification of fish CMPK2 as an interferon stimulated gene against SVCV infection. Fish & shellfish immunology. 92:125-132.
Abstract
Cytidine/uridine monophosphate kinase 2 (CMPK2) is known as a nucleoside monophosphate kinase in mitochondria to maintains intracellular UTP/CTP, and could be induced by immunostimulants LPS and Poly (I:C) in mammals, suggesting its potential antiviral and antibacterial role. In this study, CMPK2 was cloned and characterized in Fathead minnow (FHM) cells. In vivo analysis of tissue distribution revealed that CMPK2 transcript was detected in all the tissues of zebrafish (Danio rerio) examined in this study, particularly abundant in liver, spleen and kidney. In addition, indirect immunofluorescence showed that CMPK2 was localized in the cytoplasm of FHM cells. Expression of CMPK2 mRNA was significantly up-regulated following challenge with Spring viraemia of carp virus (SVCV), poly(I:C), or zebrafish IFN1 and IFN3 both in vitro and in vivo. Furthermore, overexpression and RNA interference of CMPK2 in SVCV-infected FHM cells showed significantly antiviral effect. In summary, this study for the first time shows the presence and distribution of CMPK2 in different tissues of zebrafish, but also demonstrates its antiviral potential against SVCV infection in vivo. These new findings could contribute to explain the molecular mechanism of the CMPK2 mediated antiviral function.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping