Person

Hauptmann, Giselbert

Person ID: ZDB-PERS-970128-5
Email: giselbert.hauptmann@su.se
URL
Affiliation: Hauptmann Lab
Address: Stockholm University Department of Molecular Biosciences The Wenner-Gren Institute Svante Arrhenius väg 20C Stockholm, S-10691 Sweden
Country: Sweden
Phone
Fax
ORCID ID

Biography and Research Interest

In situ mRNA detection and visualization in neural tissue

Evolution and development of vertebrate forebrain organization

Development and function of neuroendocrine hypothalamus

Development and function of corticotropin-releasing-hormone neuronal
systems

Publications

Non-Zebrafish Publications

Hauptmann, G. & Gerster, T.
"Multicolor whole-mount in situ hybridization to Drosophila embryos"
Dev. Genes Evol. 1996 Dec (206): 292-295
ABSTRACT
We report an extended whole-mount in situ hybridization procedure for Drosophila embryos. By using probes labelled with digoxigenin, fluorescein and biotin,
respectively, this protocol allows the detection in three colors of RNAs derived from three different genes. Hybridized probes are detected by consecutive
staining with appropriate alkaline phosphatase conjugates using different chromogenic substrate combinations, and serial removal of the antibody conjugates by
low pH washes.