Person
Winkler, Christoph
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Biography and Research Interest
Expression cloning of neural inducing factors in zebrafish: In a cross-species approach zebrafish cDNA libraries are screened for factors with neural inducing activity in a Xenopus animal cap assay. In vitro transcribed RNAs from pools of a zebrafish gastrula stage and adult brain library are injected into early Xenopus embryos. Animal caps are dissected at the blastula stage and cultivated until control embryos reach late neural stages. Induction of neural marker expression in the explants is assayed by RT-PCR with panneural markers as well as regionally restricted neural markers. Subsequent sib selection of positively identified pools with neural-inducing activities will finally lead to the identification of single clones that are involved in the induction and/or patterning of the early vertebrate nervous system. Those factors will be functionally characterized in the available zebrafish mutants with defects in the formation of neural structures.
Non-Zebrafish Publications
Loosli, F., Bene, F.D., Quiring, R., Rembold, M., Martinez-Morales, J.R., Carl, M., Grabher, C., Iquel, C., Krone, A., Wittbrodt, B., Winkler, S., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Henrich, T., Deguchi, T., Hirose, Y., Iwanami, N., Kunimatsu, S., Osakada, M., Watanabe, T., Yasuoka, A., Yoda, H., Winkler, C., Elmasri, H., Kondoh, H., Furutani-Seiki, M., and Wittbrodt, J. (2004) Mutations affecting retina development in Medaka. Mech. Dev.. 121(7-8):703-714Kitagawa, D., Watanabe, T., Saito, K., Asaka, S., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Yasuoka, A., Deguchi, T., Yoda, H., Hirose, Y., Henrich, T., Iwanami, N., Kunimatsu, S., Osakada, M., Winkler, C., Elmasri, H., Wittbrodt, J., Loosli, F., Quiring, R., Carl, M., Grabher, C., Winkler, S., Del Bene, F., Momoi, A., Katada, T., Nishina, H., Kondoh, H., and Furutani-Seiki, M. (2004) Genetic dissection of the formation of the forebrain in Medaka, Oryzias latipes. Mech. Dev.. 121(7-8):673-685
Elmasri, H., Winkler, C., Liedtke, D., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Henrich, T., Hirose, Y., Yasuoka, A., Yoda, H., Watanabe, T., Deguchi, T., Iwanami, N., Kunimatsu, S., Osakada, M., Loosli, F., Quiring, R., Carl, M., Grabher, C., Winkler, S., Del Bene, F., Wittbrodt, J., Abe, K., Takahama, Y., Takahashi, K., Katada, T., Nishina, H., Kondoh, H., and Furutani-Seiki, M. (2004) Mutations affecting somite formation in the Medaka (Oryzias latipes). Mech. Dev.. 121(7-8):659-671
Furutani-Seiki, M., Sasado, T., Morinaga, C., Suwa, H., Niwa, K., Yoda, H., Deguchi, T., Hirose, Y., Yasuoka, A., Henrich, T., Watanabe, T., Iwanami, N., Kitagawa, D., Saito, K., Asaka, S., Osakada, M., Kunimatsu, S., Momoi, A., Elmasri, H., Winkler, C., Ramialison, M., Loosli, F., Quiring, R., Carl, M., Grabher, C., Winkler, S., Del Bene, F., Shinomiya, A., Kota, Y., Yamanaka, T., Okamoto, Y., Takahashi, K., Todo, T., Abe, K., Takahama, Y., Tanaka, M., Mitani, H., Katada, T., Nishina, H., Nakajima, N., Wittbrodt, J., and Kondoh, H. (2004) A systematic genome-wide screen for mutations affecting organogenesis in Medaka, Oryzias latipes. Mech. Dev.. 121(7-8):647-658
Hong, Y., Winkler, C. & Schartl, M. (1998). Efficiency of cell culture derivation from blastula embryos and of chimera formation in the medaka (Oryzias latipes) depends on donor genotype and passage number. Dev Genes Evol 208, 595-602.
Dimitrijevic, N., Winkler, C., Wellbrock, C., Gomez, A., Duschl, J., Altschmied, J. & Schartl, M. (1998). Activation of the Xmrk proto-oncogene of Xiphophorus by overexpression and mutational alterations. Oncogene 16, 1681-1690.
Hong, Y., Winkler, C. & Schartl, M. (1998). Production of medakafish chimeras from a stable embryonic stem cell line. Proc. Natl. Acad. Sci. 95, 3679-3684.
Hyodo-Taguchi, Y., Winkler, C., Kurihara, Y., Schartl, A. & Schartl, M. (1997). Phenotypic rescue of the albino mutation in the medakafish (Oryzias latipes) by a mouse tyrosinase transgene. Mech. Dev. 68, 27-35.
Baudler, M., Duschl, J., Winkler, C., Schartl, M. & Altschmied, J. (1997). Activation of transcription of the melanoma inducing Xmrk oncogene by a GC box element. J. Biol. Chem. 272, 131-137.
Hong, Y., Winkler, C. & Schartl, M. (1996). Pluripotency and differentiation of embryonic stem cell lines from the medakafish (Oryzias latipes). Mech. Dev. 60, 33-44.
Winkler, C., Wittbrodt, J., Lammers, R., Ullrich, A. & Schartl, M. (1994). Ligand-dependent tumor induction in medakafish by a Xmrk receptor tyrosine kinase transgene. Oncogene 9, 1517-1525.