IMAGE

Figure 6

ID
ZDB-IMAGE-230629-65
Source
Figures for Mazumdar et al., 2023
Image
Figure Caption

Figure 6

Aberrant ethanol-induced apoptosis in nnt mutants is rescued by antioxidant treatment. (A–F) 36 hpf nnt;fli1a:eGFP (green) embryos stained with TUNEL (red) (n = 14 per group). Lateral views, anterior to the left, dorsal up. (A) Unexposed wildtypes had significantly lower apoptosis in the pharyngeal arches compared to (D) unexposed mutants (p = 0.0230). (B) 1% ethanol-dosed wildtypes had significantly less apoptosis in the arches and brain compared to (E) ethanol-treated mutants (p = <0.0001 and p = 0.0003). (C) 1 mM NAC + 1% EtOH dosed wildtypes were not significantly different from (F) NAC + EtOH-treated mutants (p = 0.7775), but NAC + EtOH mutants had significantly lower apoptosis in the arches and brain compared to ethanol-dosed mutants (p = <0001 and p = 0.0491, respectively). (G–J) Schematic of the spatial distribution of apoptosis in the brains (blue arrow) and pharyngeal arches (red arrow) of untreated and ethanol-treated mutants and wildtypes (n = 5 per group). (G) The wildtype control had fewer apoptotic cells that were more widely distributed across the brain and arches compared to the (H,I) unexposed mutants and ethanol-treated wildtypes that had higher levels of apoptosis in both regions. (J) Ethanol-treated mutants had more apoptosis than all other groups and had cell death localized to the ventral portion of the arches and clustered around the midbrain-hindbrain boundary. (K) Graph depicting apoptotic cells in the neural crest across all groups (Two-way ANOVA with multiple comparisons, black bars depict mean ± SEM, n = 14 per group, *p < 0.05, **p < 0.01, ****p < 0.0001). (L) Graph depicting apoptotic cells in the brain across all groups (Two-way ANOVA with multiple comparisons, black bars depict mean ± SEM, n = 14 per group, *p < 0.05, **p < 0.01, ***p < 0.001). Ctrl: control, EtOH: ethanol, NAC: N-acetyl Cysteine, Mut: mutant, Wt: wildtype.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neurosci.