ZFIN Image: Mazumdar et al., 2023, Figure 1

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Figure Caption

Figure 1

Generation of a zebrafish nnt mutation. (A) Schematic depicting the 74 bp deletion in exon 4 of nnt. The gRNA binding site (gRNA jke888) and PAM sequence are noted. (B) Location of the mutation (designated au111) relative to the 22 exon long gene. (C) Confocal images of fluorescent in situ hybridization staining for nnt-specific probe in 24 hpf wildtype and (D) mutant embryos, demonstrating a lack of transcript in nnt mutants, suggesting extensive non-sense mediated decay of the mutant transcript. Anterior is left, dorsal is up, e: eye, ea: ear.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neurosci.