Figure 4

Mutants in capn3b gene are significantly more sensitive than wild-type zebrafish to growth in methylcellulose. (A) Birefringence imaging of wild-type, capn3b^mut1/mut1, and capn3b^−/− (rnaless) mutant embryos after culturing them from 2 days post fertilization (dpf) to 5 dpf under control conditions or in 0.8% methylcellulose (MC) in E3 medium. Photographs of embryos with normal muscle morphology or disrupted (abnormal) are shown, except for wild-type embryos, which were almost completely resistant to the treatment. The combined numbers out of the total analyzed are shown at the lower right-hand corner of the representative images. (B) Plot of the percentages of embryos with abnormal muscle structure (abnormality percentage) for embryos of different genotypes. Significance is calculated using the Cochran–Mantel–Haenszel test using all available counts in 3-dimensional contingency tables. “***” indicates p-values < 0.001 and ‘*’—p-value < 0.05.