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Figure 6

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ZDB-IMAGE-230228-131
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Figures for Ritter et al., 2023
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Figure 6

rgp1-/- chondrocytes undergo cell death and induce a Golgi stress marker. (A) TEM images of 3 dpf WT chondrocytes showing normal cellular morphology. (B) rgp1-/- craniofacial chondrocytes showing partial nuclear condensation (yellow shading, blue arrow) and clear cytoplasmic vacuoles (blue shading, blue arrow). (C) Quantification of cell death-associated TEM phenotypes. (D) Representative images of chondrocytes labeled with TUNEL and WGA in WT and rgp1-/- cartilage (TUNEL, magenta; WGA, green). Yellow arrowhead identifies TUNEL+ nucleus. (E) Quantification of the percentage of nuclei that were TUNEL+ in each image. (F) Experimental design for mosaic overexpression of DN GFP-hRAB8A fusion protein in zebrafish chondrocytes, as used in Figure 5A . (G) Quantification of the percentage of nuclei that were TUNEL+ in each image at 3 dpf or 4 dpf (H) qPCR panel of ER stress transcripts (edem1, bip, chop) and Golgi stress transcripts (arf4a, arf4b, tfe3a, tfe3b, acbd3) normalized to ef-1 transcript levels in 3 dpf larvae. Significance is presented by *p<0.05; ****p<0.0001; ns, not signficant.

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Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)