|
Fig. 2 hoxa13;d13 mutants have posterior defects. (A) Scheme of Hox13 mutants. The coding regions of hoxa13b and hoxd13a are shown, with the DNA binding homeobox indicated. The hoxa13bΔ16 mutant is truncated due to a 16 base pair (bp) deletion that causes a frameshift, adding additional amino acids (green). Three hoxd13a mutants are shown, with insertions of 13 or 4 bp or a deletion of 8 bp. The 8 bp deletion causes an immediate truncation, whereas the insertion mutants cause frameshifts that add a small number of additional amino acids. (B) Posterior defects observed in hoxa13Δ16;hoxd13ains13 mutants (lower two embryos) compared with a wild-type embryo (top) at 3 dpf.