ZFIN Image: Tingaud-Sequeira et al., 2020, Fig. 2

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Figure Caption

Fig. 2 The nonsense variant encodes a mislocalized truncated ZYG11B protein. (a) Western blot validated overexpression experiments. (b) Immunocytochemistry detection of ZYG11B‐WT and ZYG11B‐E537* in HeLa cells. (c) RT‐qPCR experiments validated ZYG11B expression knockdown by the specific siRNA‐ZYG11B which also induced a significant down‐regulation of SOX6 expression, t test p < .05. (d) Cell proliferation is significantly decreased when treated with siRNA‐ZYG11B (Kruskal–Wallis test followed by Dunns’ post hoc multiple comparison test (p < .05)). (d) RT‐qPCR analysis of ZYG11B expression showed a down‐regulation following RA treatment in HeLa cells (t test p < .05)

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