ZFIN Image: Pini et al., 2020, Fig. EV4

Image

Figure Caption

Fig. EV4 <styled-content toggle='no' style='fixed-case'>CRISPR</styled-content>/Cas9 targeted mutagenesis of <italic>alx1</italic> in zebrafish

A

Human ALX1 and zebrafish alx1 protein sequences were obtained from Ensembl and aligned using Clustal Omega (https://www.ebi.ac.uk/Tools/msa/clustalo/) under the default settings. The homeobox DNA‐binding domain is shown in bold, with the amino acid residue mutated in the subjects indicated by an outline. The transactivation domain is shaded in gray. Zebrafish alx1 CRISPR sites #1 and #2 are highlighted in yellow. The red and blue letters visually demarcate the sites.

B

Schematic diagram shows the effect of the mutant allele resulting from our choice of target site #1. The allele, termed, alx1^uw2016, has a net deletion of 16 nucleotides. Red letters denote the abnormal sequence that results from the frameshift mutation.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.