ZFIN Image: Clark et al., 2019, Fig. 6.

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Figure Caption

Fig. 6.

Chemical ER stressors activate reporter expression. (A) Zebrafish embryos were treated with ER stressors at 1 dpf. Quantification (dashed line) revealed that 5XATF6RE:d2GFP expression was significantly higher at 8 hpt with thapsigargin (P=0.0004) and brefeldin A (P=0.0003) treatment, at 24 hpt with tunicamycin (P=0.0001) and brefeldin A (P=0.0001) treatment, and at 48 hpt with tunicamycin (P=0.0001) and brefeldin A (P=0.0065) treatment. **P≤0.01; ***P≤0.001; ****P≤0.0001; unpaired one-way ANOVA with Dunnett's post-hoc test with respect to DMSO control group. All error bars are s.e.m. (B) Representative images showing increased 5XATF6RE:d2GFP expression in the spinal cord of embryos (at 48 hpt) treated with tunicamycin or brefeldin A compared to the DMSO-treated control. Scale bars: 1 mm (A); 0.1 mm (B).

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Acknowledgments

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