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Figure 6

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ZDB-IMAGE-200130-20
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Figures for Chen et al., 2019
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Figure Caption

Figure 6

LPA3 protects fibroblasts against cell senescence caused by oxidative stress. (a) CCK‐8 assay showed that knockdown of LPA receptors alone had no effect on cell proliferation. (b) CCK‐8 revealed that treatment with 10 μM and 50 μM H2O2 for 3 days significantly decreased cell proliferation in knockdown of LPA3, but not of LPA1 or LPA2. CCK‐8 reads were normalized to a sham group. (c) Representative images of senescence‐associated β‐gal staining assay. Treatment with 10 μM and 50 μM H2O2 for 3 days significantly increased percentages of β‐gal + cells in knockdown of LPA3, but not of LPA1 or LPA2. Quantification was shown in (d). (e) Western blot showed that 100 μM of H2O2 treatment for 6 hr increased protein level of Nrf2, NQO1, SOD2, and Gpx1, whereas LPA3 knockdown abolished the effects. (f) Graphical abstract of this study. ANOVA and Student's t test; *p < .05, **p < .01, ***p < .001

Acknowledgments
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