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Fig. 3 Expression and activity of mutant miRNAs. (a–c) Wild-type and mutant miRNAs were detected in adult zebrafish retina by qPCR. Mutant animals were all homozygotes. Asterisks indicate significantly decreased expression, relative to WT. nd = not detected. (d–f) miRNAs were cloned from adult mutant and wildtype genomic DNA and co-transfected with luciferase reporters encoding miRNA binding sites (MBS) for the indicated miRNAs. Decreased reporter signal indicates miRNA activity, relative to the miR-129 control miRNA. RLU = relative light units. **p < 0.01, ***p < 0.001, ****p < 0.0001, ANOVA with Dunnet’s multiple comparisons test. For all data points, n = 3.