ZFIN Image: Schepis et al., 2018, Fig. 5

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Fig. 5

Altered distribution of nuclei and E-cadherin in basal-layer keratinocytes in hai1a morphants and its par2b-dependence. Control, hai1a morphant, and par2b^−/− hai1a morphant periderm-nuclear-GFP embryos were collected at 30 hpf, immunostained for the basal layer marker p63 and E-cadherin, and imaged by confocal fluorescence microscopy. (A) Nuclear-GFP marker of periderm cells. Note regular spacing in controls (left), clustered and crowded appearance in hai1a morphants (middle), and increased number in par2b^−/− hai1a morphants (right). (B) p63 marker for basal layer nuclei. Note regular spacing in controls (left), clustered and crowded appearance in hai1a morphants (middle), and more control-like pattern in par2b^−/− hai1a morphants (right). (C) E-cadherin staining in basal layer. Note localization to cell–cell junctions in controls (left), aberrant distribution with increased cytosolic staining in areas coinciding with clusters of p63-positive nuclei in hai1a morphants (middle), and more control-like pattern in par2b^−/− hai1a morphants (right). Bar, 20 µm.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Biol.