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Fig. 2

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ZDB-IMAGE-180420-18
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Figures for Silva et al., 2017
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Fig. 2

MTHs regulate the WNT and NOTCH pathway genes during zebrafish embryogenesis. (A) WISH expression analysis of wnt1. Lateral (upper panel) and dorsal images of the brain (lower panel) in control and MCT8 morphant zebrafish embryos at 25hpf. (B) WISH expression of the WNT1 protein secretion regulator - wls, lateral and dorsal images of the brain and lateral images of the spinal cord in control and MCT8 morphant zebrafish embryos at 25hpf. The red arrows denote wls - expressing cells in the spinal cord. (C) Fluorescent WIHC expression analysis of the NOTCH ligand DeltaA in the hindbrain and spinal cord of control and MCT8 morphant embryos at 25hpf. (D) WISH expression analysis of the NOTCH pathway target gene her2 in control and MCT8 morphant zebrafish embryos at 25hpf. Lateral and dorsal images of the brain (first and second panels) and lateral images of the spinal cord are shown (lower panel). hb-hindbrain; mb-midbrain; nt-notochord. In (A,B,D) the scale bars represent 100 μm. In (C) the scale bars represents 25 μm.

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