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Fig. 2 S1
Exogenous PGB2 treatment is sufficient to expand the proximal straight tubule.
(A) Embryos were treated with PGB2 at 100 μM from 4 hpf to 24 hpf. Using WISH, embryos were stained for the PCT (slc20a1a), PST (trpm7), DE (slc12a1), and DL (slc12a3) (purple) and the somites (smyhc1) (red) at the 24 hpf stage. Black bars indicate segment gene expression domain. Red scale bar, 70 µm. (B) Summary depicting the nephron segments after PGB2 treatment at 100 μM.
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