ZFIN Image: Davey et al., 2016, Fig. S2

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Figure Caption

Fig. S2

Post-mitotic FBMNs require PCP signaling for migration.

(A) Live confocal image showing the dorsal view of a pk1b mutant embryo hindbrain at 48 hpf after transplantation of post-mitotic FBMNs from a wild type donor. Cascade blue-dextran marks all donor-derived cells (blue), Tg(isl1:GFP) marks host FBMNs (green) and Tg(isl1:mRFP) marks donor-derived FBMNs (magenta). (B) Histogram indicates the percent of donor-derived FBMNs at 48 hpf that failed to migrate, (rhombomere (r)4), partially migrated (r5) or fully migrated (r6) and numbers indicate the number of FBMNs represented in each bar. White arrows indicate migrated donor derived FBMNs. While post-mitotic FBMNs in general migrate poorly after being transplanted, they do sometimes migrate in WT and pk1b mutant hosts but never in vangl2 mutant hosts (see Fig 2). Brackets indicate rhombomere positon. Scale bar: 50μm.

Acknowledgments

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