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Fig. 4
EYS-deficient zebrafish exhibited degeneration of cone photoreceptors. Zebrafish retinal sections were immunostained with GNAT2, 1D4, green opsin, blue opsin, and UV opsin antibodies. (A-E′) GNAT2, 1D4, green opsin, blue opsin, and UV opsin staining of 40 dpf wild-type and EYS-deficient zebrafish. There were no apparent differences between wild-type and EYS-deficient zebrafish. (F-J′) GNAT2, 1D4, green opsin, blue opsin, and UV opsin staining of 6 mpf wild-type and EYS-deficient zebrafish. Note GNAT2-, 1D4-, green opsin-, blue opsin-, and UV opsin-positive immunoreactivity was reduced in EYS-deficient zebrafish. (K-O′) GNAT2, 1D4, green opsin, blue opsin, and UV opsin staining of 8 mpf wild-type and EYS-deficient zebrafish. Note that GNAT2-, 1D4-, green opsin-, blue opsin-, and UV opsin-positive immunoreactivity was further reduced in EYS-deficient zebrafish. Scale bar: 20 µm.