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Fig. 8
Morphological phenotype of embryos injected with fdx1 morpholino at 9 hpf.
A, Embryos injected with fdx1-ATGMO, fdx1-SplMO, or fdx1-CtlMO were classified based on their progression of epiboly at 9 hpf. Class I represents a normal epiboly, and class II and class III represent delayed movement as indicated by the black arrow. B, Injection of 9 ng of fdx1-CtlMO showed 95% of the normal class I phenotype compared with fdx1-ATGMO-injected embryos, which had 68.9% class II and 18% class III. Coinjection of either fdx1 or fdx1b mRNA with fdx1-ATGMO partially restored embryos to a class I phenotype.
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