ZFIN Image: Tessadori et al., 2015, Fig. 2

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Figure Caption

Fig. 2

FurinA Is Required Cell-Autonomously to Process Spaw and to Confer Its Long-Range Signaling Activity

(A) Schematic of the transplantation procedure.

(B and C) Both MZspaw^-/- (B) and MZaoh^-/- (C) embryos fail to express spaw in the left LPM at 18 somites.

(D) ISH for spaw (blue staining) with immunolabeling for transplanted cells (red staining). Transplantation of spaw-injected spaw^-/- cells (yellow clone, red outline) in MZspaw^-/- resulted in the rescue of spaw expression in the left LPM several cell diameters away from the transplanted clone.

(E) Transplantation of cells injected with RNA of a noncleavable form of spaw (yellow clone, purple outline) in MZspaw^-/- failed to induce of spaw expression in the left LPM.

(F) Transplantation of spaw-injected spaw^-/- cells (yellow clone, red outline) in MZaoh^-/- resulted in induction of spaw expression in the left LPM several cell diameters away from the transplanted clone.

(G) Spaw-injected aoh^-/- cells (green clone, red outline) in MZspaw^-/- were unable to induce spaw expression in the left LPM. In all schematics, MZspaw^-/- cells appear in yellow and MZaoh^-/- cells in green.

Figure Data

Expression / Labeling details

Acknowledgments

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Reprinted from Developmental Cell, 32(5), Tessadori, F., Noël, E.S., Rens, E.G., Magliozzi, R., Evers-van Gogh, I.J., Guardavaccaro, D., Merks, R.M., Bakkers, J., Nodal Signaling Range Is Regulated by Proprotein Convertase-Mediated Maturation, 631-9, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell