ZFIN Image: Tessadori et al., 2015, Fig. S4

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Figure Caption

Fig. S4 Overexpression of FurinA perturbs the laterality of Spaw expression. Wt and Zaoh mutant embryos injected at the 1-cell stage with furinA mRNA were assayed for spaw expression by ISH at 16 somites (17 hpf) and 20 somites (19 hpf). The observed expression patterns were separated in classes presented in (A) and quantified for each condition in (B) and (C). At 16 somites (B), the expected pattern of expression is “middle” in normally developing wt embryos, while it is mainly “low” in Zaoh. Additionally to the increased expansion of the spaw expression domain, note that overexpression of furinA results in the appearance of bilateral and right-sided expression of spaw. (C) At 20 somites, the expected pattern of expression is “high” in wt embryos.

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Reprinted from Developmental Cell, 32(5), Tessadori, F., Noël, E.S., Rens, E.G., Magliozzi, R., Evers-van Gogh, I.J., Guardavaccaro, D., Merks, R.M., Bakkers, J., Nodal Signaling Range Is Regulated by Proprotein Convertase-Mediated Maturation, 631-9, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell