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Fig. S1 Knockdown Efficiency of Morpholinos, Related to Figure 2.(A) Immunohistochemistry of clock gene mRNA-injected zebrafish embryos with or without morpholinos. The translated proteins were detected using an anti-myc tag antibody, followed by detection with an alkaline phosphatase-conjugated secondary antibody. The translated positive signals were revealed using the NBT/BCIP technique. Blue color shows positive signals of the translated proteins.(B) Quantifications of average clock protein levels ± SEM in mRNA-injected zebrafish embryos (18-24 embryos/group).
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