ZFIN Image: Du et al., 2008, Fig. 5

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Figure Caption

Fig. 5

Knockdown of Hsp90α1 results in MHC degradation and up-regulation of unc-45b expression. (A) Western blot analysis shows the low levels of MHC proteins in Hsp90α1 knockdown embryos. In contrast, expression levels of actin and myogenin were not affected. (B) RT-PCR analysis of fast-muscle MHC (fMHC), slow-muscle MHC (sMHC), unc-45b, Hsp90α1, and ef-1α expression in control (lanes 1, 3, 5, 7, and 9) or Hsp90α1 ATG-MO injected (lanes 2, 4, 6, 8, and 10) embryos. (C–F) Side views (C and E) or cross-sections (D and F) show unc-45b expression in control (C and D) or Hsp90α1 ATG-MO (E and F) injected embryos at 24 hpf.

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Expression / Labeling details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA