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Fig. 6 RA signaling is essential for regenerative outgrowth and maintenance of the regenerate. Inhibition of RA signaling in hsp70:cyp26a1 fish during regenerative outgrowth blocks further regeneration and abolishes maintenance of the regenerate (daily heat shocks, first heat shock at 72 hpa). (A) Before the first heat shock, regeneration in hsp70:cyp26a1 fish is indistinguishable from that of wild-type fish. (B) Ten hours after the first heat shock, the blastema of hsp70:cyp26a1 fish regenerates appears dark. (C) Two days later, regenerative outgrowth is blocked and already regenerated tissue is lost. Note the decrease in tissue distal to the amputation plane in hsp70:cyp26a1 regenerates between A and C. Dashed lines indicate amputation plane. hs, heat shock. Scale bar: 200 μm.