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Fig. 2 Id2a is required for the differentiation of late-born retinal neurons and Müller glia. Immunohistochemical analysis of markers (red) of neuronal and glial differentiation in Id2a-MM and Id2a-MO zebrafish embryos at 72 hpf. (A,B) Ganglion cells (zn8), (C,D) amacrine cells (5e11), (E,F) bipolar cells (PKC), (G,H) Müller glia (zrf1), (I,J) rods (zpr3) and (K,L) red/green cones (zpr1). Although Id2a-MO retinas contain ganglion cells (B) and an occasional rod cell (J, arrow) or Müller glia cell (H, arrow), all late differentiated cell types are absent. DNA, green.
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