ZFIN Image: Lee et al., 2006, Fig. 2

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Figure Caption

Fig. 2 Comparative analysis of the mesenchymal and ectodermal components of blimp-1 expression with respect to tbx5 and dlx2a using double label fluorescent in situ hybridization. (A) A wild-type embryo, showing blimp-1 expression in the fin bud. (B) The same embryo showing tbx5 expression in the fin bud. The ectoderm, which is devoid of tbx5 transcripts, is indicated (arrows). (C) Superimposition of the images depicted in panels A and B, showing colocalization of blimp-1 and tbx5 signals in the mesenchyme, while ectodermal cells only contain blimp-1 transcripts (arrows). (D-F) blimp-1 and dlx2a are co-expressed in cells of the AER from 30 hpf (arrow). All panels depict lateral views.

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Acknowledgments

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Reprinted from Developmental Biology, 300(2), Lee, B.C., and Roy, S., Blimp-1 is an essential component of the genetic program controlling development of the pectoral limb bud, 623-634, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.