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Fig. S4 Genetic Interactions between tob1a and lef1, tcf3 (A) Interaction between Tob1a and the β-Catenin coactivator Lef1. Single-cell embryos were injected with 400 pg tob1a mRNA, 300 pg lef1 mRNA or both, and examined for the expression of the dorsal markers bozozok (boz) and chordin (chd). Injection with lef1 mRNA alone caused no obvious changes. Decrease or elimination of boz and chd was seen in 66% (n=32), 61% (n=28) of embryos injected with tob1a mRNA alone, but only in 19% (n=36) and 21% (n=33) of embryos coinjected with tob1a and lef1 mRNA, respectively. This suggests that overexpression of lef1 alleviated inhibitory effect of tob1a overexpression. Upper panel, lateral views with the dorsal to right at the 30% epiboly stage; lower panel, animal-pole views with the dorsal to right at the shield stage. (B) Interaction between Tob1a and the dominant negative form (Tcf3ΔN) of the β-Catenin coactivator Tcf3. Injection with 20 ng tob1a-MO caused an increase of chd in 81% of embryos (n=26). Injection with 200 pg tcf3ΔN mRNA resulted in elimination or decrease of chd expression in 90% of embryos (n=29). When tcf3ΔN mRNA and tob1a-MO were coinjected, 88% (n=33) of embryos still showed a decrease of chd expression. Embryos at the shield stage were animal-pole views with the dorsal to right.
Reprinted from Developmental Cell, 11(2), Xiong, B., Rui, Y., Zhang, M., Shi, K., Jia, S., Tian, T., Yin, K., Huang, H., Lin, S., Zhao, X., Chen, Y., Chen, Y.G., Lin, S.C., and Meng, A., Tob1 controls dorsal development of zebrafish embryos by antagonizing maternal beta-catenin transcriptional activity, 225-238, Copyright (2006) with permission from Elsevier. Full text @ Dev. Cell