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Fig. 8

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ZDB-IMAGE-070621-8
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Figures for Cheng et al., 2006
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Fig. 8 The effect of irx1a knockdown on shh-GFP expression is both cell-autonomous and non-cell-autonomous. Lateral view of 48 hpf retina. Ventral to the bottom, anterior to the left. Blastomere transplantations were carried out to determine the cell-autonomy of the effects of irx1a knockdown. The donor embryos, which carry the shh-GFP transgene, were labeled with rhodamine-dextran. Donor cells were transplanted into wild-type shh-GFP hosts at blastula stages. The shh-GFP expression in retinas with transplanted irx1a-MO cells was severely reduced (D–I), while control-MO injected donor cells had no effect on shh-GFP expression (A–C). Transplanted cells failed to express the shh-GFP transgene in all cases (red stain panels D–F) suggesting a cell-autonomous requirement of irx1a. However, shh-GFP expression was also reduced in areas that were not occupied by irx1a-MO containing cells. Host retinas with large irx1a-MO clones (G–I) lacked almost entirely GFP expression in areas derived from host cells. These results indicate an additional non-cell-autonomous effect of irx1a knockdown. A microphthalmia phenotype was also observed in the irx1a-MO transplanted hosts with large clones of irx1a-MO cells.

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Reprinted from Mechanisms of Development, 123(3), Cheng, C.W., Yan, C.H., Hui, C.C., Strähle, U., and Cheng, S.H., The homeobox gene irx1a is required for the propagation of the neurogenic waves in the zebrafish retina, 252-263, Copyright (2006) with permission from Elsevier. Full text @ Mech. Dev.