CRISPR

CRISPR1-lamb1a

ID
ZDB-CRISPR-230623-1
Name
CRISPR1-lamb1a
Previous Names
None
Target
Sequence
5' - GGATCCTCAATCCTGAAGGC - 3'
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "AGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
sh589 lamb1a
sh590 lamb1a
Expression
Gene expression in Wild Types + CRISPR1-lamb1a
No data available
Phenotype
Phenotype resulting from CRISPR1-lamb1a
No data available
Phenotype of all Fish created by or utilizing CRISPR1-lamb1a
Phenotype Fish Conditions Figures
cardiac ventricle myh7l expression increased amount, abnormal lamb1ash590/sh590 standard conditions Fig. 3 with image from Derrick et al., 2021
endocardium aldh1a2 expression increased distribution, abnormal lamb1ash590/sh590 standard conditions Fig. 7 with image from Derrick et al., 2021
atrial endocardium klf2a expression increased amount, abnormal lamb1ash590/sh590 standard conditions Fig. 5 with image from Derrick et al., 2021
atrium myh6 expression increased amount, abnormal lamb1ash590/sh590 standard conditions Fig. 3 with image from Derrick et al., 2021
endocardium lamb1b expression mislocalised, abnormal lamb1ash590/sh590 standard conditions Fig. 5 with image from Derrick et al., 2021
endocardium lamb1b expression increased distribution, abnormal lamb1ash590/sh590 standard conditions Fig. 5 with image from Derrick et al., 2021
heart myl7 expression increased amount, abnormal lamb1ash590/sh590 standard conditions Fig. 2 with imageFig. 6 with image from Derrick et al., 2021
endocardium aldh1a2 expression mislocalised, abnormal lamb1ash590/sh590 standard conditions Fig. 7 with image from Derrick et al., 2021
ventricular endocardium klf2a expression increased amount, abnormal lamb1ash590/sh590 standard conditions Fig. 5 with image from Derrick et al., 2021
heart myl7 expression spatial pattern, abnormal lamb1ash590/sh590 standard conditions Fig. 2 with image from Derrick et al., 2021
atrium cell DsRed2 expression increased amount, abnormal lamb1ash590/sh590; f2Tg standard conditions Fig. 3 with image from Derrick et al., 2021
heart proximal side EGFP expression increased amount, abnormal lamb1ash590/sh590; f2Tg; twu34Tg standard conditions Fig. 4 with image from Derrick et al., 2021
atrial endocardium DsRed2 expression increased amount, abnormal lamb1ash590/sh590; f2Tg; twu34Tg standard conditions Fig. 6 with image from Derrick et al., 2021
heart distal side EGFP expression increased amount, abnormal lamb1ash590/sh590; f2Tg; twu34Tg standard conditions Fig. 4 with image from Derrick et al., 2021
heart proximal side DsRed2 expression increased amount, abnormal lamb1ash590/sh590; f2Tg; twu34Tg standard conditions Fig. 4 with image from Derrick et al., 2021
regulation of secondary heart field cardioblast proliferation increased process quality, abnormal lamb1ash590/sh590; f2Tg; twu34Tg standard conditions Fig. 4 with imageFig. 6 with image from Derrick et al., 2021
atrial endocardium EGFP expression increased amount, abnormal lamb1ash590/sh590; f2Tg; twu34Tg standard conditions Fig. 6 with image from Derrick et al., 2021
heart distal side DsRed2 expression increased amount, abnormal lamb1ash590/sh590; f2Tg; twu34Tg standard conditions Fig. 4 with image from Derrick et al., 2021
atrium DsRed2 expression increased amount, abnormal lamb1ash590/sh590; f2Tg; twu34Tg standard conditions Fig. 4 with image from Derrick et al., 2021
atrium EGFP expression increased amount, abnormal lamb1ash590/sh590; f2Tg; twu34Tg standard conditions Fig. 4 with image from Derrick et al., 2021
ventricular endocardium lamb1b expression decreased amount, abnormal lamb1ash590/+ + MO1-tnnt2a + MO4-tp53 standard conditions Fig. 5 with image from Derrick et al., 2021
endocardium klf2a expression decreased amount, abnormal lamb1ash590/+ + MO1-tnnt2a + MO4-tp53 standard conditions Fig. 5 with image from Derrick et al., 2021
atrial endocardium lamb1b expression decreased amount, abnormal lamb1ash590/+ + MO1-tnnt2a + MO4-tp53 standard conditions Fig. 5 with image from Derrick et al., 2021
endocardium klf2a expression amount, ameliorated lamb1ash590/sh590 + MO1-tnnt2a + MO4-tp53 standard conditions Fig. 5 with image from Derrick et al., 2021
ventricular endocardium lamb1b expression decreased amount, abnormal lamb1ash590/sh590 + MO1-tnnt2a + MO4-tp53 standard conditions Fig. 5 with image from Derrick et al., 2021
heart myl7 expression amount, ameliorated lamb1ash590/sh590 + MO1-tnnt2a + MO4-tp53 standard conditions Fig. 6 with image from Derrick et al., 2021
endocardium aldh1a2 expression amount, ameliorated lamb1ash590/sh590 + MO1-tnnt2a + MO4-tp53 standard conditions Fig. 7 with image from Derrick et al., 2021
atrial endocardium lamb1b expression decreased amount, abnormal lamb1ash590/sh590 + MO1-tnnt2a + MO4-tp53 standard conditions Fig. 5 with image from Derrick et al., 2021
heart myl7 expression increased amount, abnormal lamb1ash590/sh590 + MO4-tp53 standard conditions Fig. 6 with image from Derrick et al., 2021
endocardium lamb1b expression increased distribution, abnormal lamb1ash590/sh590 + MO4-tp53 standard conditions Fig. 5 with image from Derrick et al., 2021
endocardium aldh1a2 expression mislocalised, abnormal lamb1ash590/sh590 + MO4-tp53 standard conditions Fig. 7 with image from Derrick et al., 2021
atrial endocardium klf2a expression increased amount, abnormal lamb1ash590/sh590 + MO4-tp53 standard conditions Fig. 5 with image from Derrick et al., 2021
ventricular endocardium klf2a expression increased amount, abnormal lamb1ash590/sh590 + MO4-tp53 standard conditions Fig. 5 with image from Derrick et al., 2021
endocardium lamb1b expression mislocalised, abnormal lamb1ash590/sh590 + MO4-tp53 standard conditions Fig. 5 with image from Derrick et al., 2021
endocardium aldh1a2 expression increased distribution, abnormal lamb1ash590/sh590 + MO4-tp53 standard conditions Fig. 7 with image from Derrick et al., 2021
regulation of secondary heart field cardioblast proliferation normal process quality, ameliorated lamb1ash590/sh590; f2Tg; twu34Tg + MO1-tnnt2a + MO4-tp53 standard conditions Fig. 6 with image from Derrick et al., 2021
atrial endocardium DsRed2 expression amount, ameliorated lamb1ash590/sh590; f2Tg; twu34Tg + MO1-tnnt2a + MO4-tp53 standard conditions Fig. 6 with image from Derrick et al., 2021
atrial endocardium EGFP expression amount, ameliorated lamb1ash590/sh590; f2Tg; twu34Tg + MO1-tnnt2a + MO4-tp53 standard conditions Fig. 6 with image from Derrick et al., 2021
atrial endocardium EGFP expression increased amount, abnormal lamb1ash590/sh590; f2Tg; twu34Tg + MO4-tp53 standard conditions Fig. 6 with image from Derrick et al., 2021
regulation of secondary heart field cardioblast proliferation increased process quality, abnormal lamb1ash590/sh590; f2Tg; twu34Tg + MO4-tp53 standard conditions Fig. 6 with image from Derrick et al., 2021
atrial endocardium DsRed2 expression increased amount, abnormal lamb1ash590/sh590; f2Tg; twu34Tg + MO4-tp53 standard conditions Fig. 6 with image from Derrick et al., 2021
Citations