CRISPR

CRISPR1-sdhb

ID
ZDB-CRISPR-210716-2
Name
CRISPR1-sdhb
Previous Names
None
Target
Sequence
5' - GTTCACCGGCCCGCCGTCA - 3'
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
None
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
rmc200 sdhb
Expression
Gene expression in Wild Types + CRISPR1-sdhb
No data available
Phenotype
Phenotype resulting from CRISPR1-sdhb
No data available
Phenotype of all Fish created by or utilizing CRISPR1-sdhb
Phenotype Fish Conditions Figures
whole organism reactive oxygen species increased amount, abnormal sdhbrmc200/rmc200 (AB) standard conditions Figure 1 with image from Dona et al., 2021
swim bladder absent, abnormal sdhbrmc200/rmc200 (AB) standard conditions Fig. 4 from Dona et al., 2020
locomotory behavior process quality, abnormal sdhbrmc200/rmc200 (AB) altered light dark cycle Fig. 5 from Dona et al., 2020
startle response decreased process quality, abnormal sdhbrmc200/rmc200 (AB) control Figure 5 with image from Dona et al., 2021
intestinal mucosa morphology, abnormal sdhbrmc200/rmc200 (AB) standard conditions Fig. S3 from Dona et al., 2020
whole organism lactate increased amount, abnormal sdhbrmc200/rmc200 (AB) standard conditions Fig. 6 from Dona et al., 2020
mitochondrial electron transport, succinate to ubiquinone process quality, abnormal sdhbrmc200/rmc200 (AB) standard conditions Fig. 6 from Dona et al., 2020
whole organism succinate increased amount, abnormal sdhbrmc200/rmc200 (AB) standard conditions Fig. 6 from Dona et al., 2020
whole organism asparagine decreased amount, abnormal sdhbrmc200/rmc200 (AB) standard conditions Fig. 6 from Dona et al., 2020
whole organism dead, abnormal sdhbrmc200/rmc200 (AB) standard conditions Fig. 3 from Dona et al., 2020
whole organism aspartate decreased amount, abnormal sdhbrmc200/rmc200 (AB) standard conditions Fig. 6 from Dona et al., 2020
whole organism decreased life span, exacerbated sdhbrmc200/rmc200 (AB) chemical treatment by environment: L-ascorbic acid Figure 3 with image from Dona et al., 2021
swim bladder decreased area, abnormal sdhbrmc200/rmc200 (AB) standard conditions Fig. 4 from Dona et al., 2020
swim bladder uninflated, abnormal sdhbrmc200/rmc200 (AB) standard conditions Fig. 4 from Dona et al., 2020
heart decreased functionality, abnormal sdhbrmc200/rmc200 (AB) standard conditions Fig. 5 from Dona et al., 2020
whole organism decreased life span, abnormal sdhbrmc200/rmc200 (AB) standard conditions Figure 3 with image from Dona et al., 2021
Fig. 3 from Dona et al., 2020
startle response decreased process quality, exacerbated sdhbrmc200/rmc200 (AB) chemical treatment by environment: L-ascorbic acid Figure 5 with image from Dona et al., 2021
goblet cell increased amount, abnormal sdhbrmc200/rmc200 (AB) standard conditions Fig. S3 from Dona et al., 2020
startle response decreased process quality, abnormal sdhbrmc200/+ (AB) chemical treatment by environment: L-ascorbic acid Figure 5 with image from Dona et al., 2021
swim bladder decreased area, abnormal sdhbrmc200/+ (AB) standard conditions Fig. 4 from Dona et al., 2020
Citations