CRISPR

CRISPR2-pgk1

ID
ZDB-CRISPR-210223-2
Name
CRISPR2-pgk1
Previous Names
None
Target
Sequence
5' - AAGGAAAGCGGGTGATCATG - 3'
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The PAM site was "AGG" at the 3' end.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
x55 pgk1
Expression
Gene expression in Wild Types + CRISPR2-pgk1
No data available
Phenotype
Phenotype resulting from CRISPR2-pgk1
No data available
Phenotype of all Fish created by or utilizing CRISPR2-pgk1
Phenotype Fish Conditions Figures
otic vesicle etv5b expression decreased amount, abnormal pgk1x55/x55 control Figure 4 with imageFigure 6 with image from Kantarci et al., 2020
otic vesicle pax5 expression decreased amount, abnormal pgk1x55/x55 control Figure 7 with image from Kantarci et al., 2020
otic vesicle neurog1 expression amount, ameliorated pgk1x55/x55 chemical treatment by environment: lactate Figure 6 with image from Kantarci et al., 2020
otic vesicle etv5b expression amount, ameliorated pgk1x55/x55 chemical treatment by environment: lactate Figure 6 with image from Kantarci et al., 2020
otic vesicle etv5b expression decreased amount, abnormal pgk1x55/x55 chemical treatment by environment: ATP Figure 6 with image from Kantarci et al., 2020
otic vesicle neurog1 expression decreased amount, abnormal pgk1x55/x55 chemical treatment by environment: ATP Figure 6 with image from Kantarci et al., 2020
otic vesicle neurod1 expression decreased amount, abnormal pgk1x55/x55 control Figure 4 with image from Kantarci et al., 2020
otic vesicle neurog1 expression decreased amount, abnormal pgk1x55/x55 control Figure 4 with imageFigure 6 with image from Kantarci et al., 2020
statoacoustic (VIII) ganglion posterior region has fewer parts of type neuron, abnormal pgk1x55/x55; zc7Tg control Figure 4 with image from Kantarci et al., 2020
statoacoustic (VIII) ganglion anterior region has fewer parts of type neuron, abnormal pgk1x55/x55; zc7Tg control Figure 4 with image from Kantarci et al., 2020
hair cell posterior macula decreased amount, abnormal pgk1x55/x55; zc7Tg control Figure 4 with image from Kantarci et al., 2020
hair cell anterior macula decreased amount, abnormal pgk1x55/x55; zc7Tg control Figure 4 with image from Kantarci et al., 2020
statoacoustic (VIII) ganglion has fewer parts of type neuron, abnormal pgk1x55/x55; zc7Tg control Figure 4 with imageFigure 5 from Kantarci et al., 2020
statoacoustic (VIII) ganglion has fewer parts of type neuron, abnormal pgk1x55/x55; zc7Tg heat shock Figure 4 - figure supplement 4 from Kantarci et al., 2020
statoacoustic (VIII) ganglion has normal numbers of parts of type neuron, ameliorated pgk1x55/x55; zc7Tg chemical treatment by environment: lactate Figure 5 from Kantarci et al., 2020
statoacoustic (VIII) ganglion has fewer parts of type neuron, abnormal pgk1x55/x55; zc7Tg chemical treatment by environment: 2-deoxy-D-glucose Figure 5 from Kantarci et al., 2020
otic vesicle pax5 expression spatial pattern, abnormal pgk1x55/x55; x17Tg heat shock Figure 7 with image from Kantarci et al., 2020
otic vesicle pax5 expression increased distribution, abnormal pgk1x55/x55; x17Tg heat shock Figure 7 with image from Kantarci et al., 2020
statoacoustic (VIII) ganglion has fewer parts of type neuron, abnormal pgk1x55/x55; x66Tg; zc7Tg heat shock Figure 4 - figure supplement 4 from Kantarci et al., 2020
statoacoustic (VIII) ganglion has fewer parts of type neuron, abnormal pgk1x55/x55; zc7Tg + MO1-plg + MO4-tp53 control Figure 4 - figure supplement 6 from Kantarci et al., 2020
statoacoustic (VIII) ganglion has fewer parts of type neuron, abnormal pgk1x55/x55; zc7Tg + MO2-plg + MO4-tp53 control Figure 4 - figure supplement 6 from Kantarci et al., 2020
Citations