CRISPR

CRISPR1-rfx4

ID
ZDB-CRISPR-180801-2
Name
CRISPR1-rfx4
Previous Names
None
Target
Sequence
5' - TCTCAGGCGTCCTCATGCCC - 3'
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
None
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
uw8013 rfx4
uw8110 rfx4
Expression
Gene expression in Wild Types + CRISPR1-rfx4
No data available
Phenotype
Phenotype resulting from CRISPR1-rfx4
No data available
Phenotype of all Fish created by or utilizing CRISPR1-rfx4
Phenotype Fish Conditions Figures
hindbrain dorsal region wnt1 expression decreased amount, abnormal rfx4uw8013/uw8013 standard conditions Fig. 6 with image from Sedykh et al., 2017
hindbrain dorsal region zic2a expression decreased amount, abnormal rfx4uw8013/uw8013 standard conditions Fig. 3 with image from Sedykh et al., 2017
spinal cord zic2a expression decreased amount, abnormal rfx4uw8013/uw8013 standard conditions Fig. 3 with image from Sedykh et al., 2017
roof plate cell disorganized, abnormal rfx4uw8013/uw8013 standard conditions Fig. 5 with image from Sedykh et al., 2017
whole organism decreased life span, abnormal rfx4uw8013/uw8013 standard conditions Fig. 2 with image from Sedykh et al., 2017
hindbrain dorsal region atoh1a expression decreased amount, abnormal rfx4uw8013/uw8013 standard conditions Fig. 6 with image from Sedykh et al., 2017
floor plate foxa2 expression spatial pattern, abnormal rfx4uw8013/uw8013 standard conditions Fig. 6 with image from Sedykh et al., 2017
trunk curved ventral, abnormal rfx4uw8013/uw8013 standard conditions Fig. 2 with image from Sedykh et al., 2017
swim bladder uninflated, abnormal rfx4uw8013/uw8013 standard conditions Fig. 2 with image from Sedykh et al., 2017
hindbrain dorsal region wnt3a expression decreased amount, abnormal rfx4uw8013/uw8013 standard conditions Fig. 3 with image from Sedykh et al., 2017
floor plate spinal cord region shha expression decreased amount, abnormal rfx4uw8013/uw8013 standard conditions Fig. 4 with image from Sedykh et al., 2017
roof plate cell amount, abnormal rfx4uw8013/uw8013 standard conditions Fig. 5 with image from Sedykh et al., 2017
whole organism dead, abnormal rfx4uw8013/uw8013 standard conditions Fig. 2 with image from Sedykh et al., 2017
floor plate spinal cord region ptch2 expression decreased amount, abnormal rfx4uw8013/uw8013 standard conditions Fig. 4 with image from Sedykh et al., 2017
midbrain dorsal region wnt1 expression decreased amount, abnormal rfx4uw8013/uw8013 standard conditions Fig. 6 with image from Sedykh et al., 2017
floor plate spinal cord region foxa2 expression decreased amount, abnormal rfx4uw8013/uw8013 standard conditions Fig. 6 with image from Sedykh et al., 2017
Citations